Contact an Account ManagerTwo-dimensional column liquid chromatographic technique for resolution of complex mixtures e.g. plant extracts
Summary
Type: HPLC
Application #: 102620
Catalog#: 720014.40 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4x250mm
Detection: UV, 254nm
Conditions: eluent A: acetonitrile
eluent B: 0.01N aqueous NaHCO3
seven gradient steps:
step 1. 1.5% A before injection
step 2. 4.2% A, 5min
step 3. 6% A, 6min 20sec
step 4. 7.5% A, 3min 30sec
step 5. 10% A, 5min
step 6. 12.5% A, 6min 20sec
step 7. 50% A, 2min 30sec
flow rate 2ml/min
Authors: Erni F
Source: J Chromatogr 149 (1978) 561 - 569
Details
Legend: Fig. 6. Reversed phase separation of a Senna glycoside extract, Peaks A and B =
sennosides A and B, respectively.